Concerning the mechanism of ester hydrolysis by proteases.

نویسندگان

  • C E Stauffer
  • E Zeffren
چکیده

We have shown conclusively that during the hydrolysis of N-acylamino acid esters by proteases the substrate bond cleaved is the acyl-carbon-ethereal oxygen bond. This was done by hydrolyzing the ethyl esters in H2180-enriched water, isolating the ethanol formed, and determining the rsO:r60 ratio. The amount of ethanol-l80 found was that of natural abundance. This result supports what has hitherto been an assumption as to the site of bond cleavage. We discuss the implications of this for various “rack,” or bond angle distortion, mechanisms which have been proposed.

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عنوان ژورنال:
  • The Journal of biological chemistry

دوره 245 13  شماره 

صفحات  -

تاریخ انتشار 1970